Mesp1-Cre knock-in mice, in which Cre recombinase is expressed under the control of the endogenous promoter-enhancer. By crossing with a mouse strain with a reporter gene like a GFP and LacZ which is interrupted by a floxed CAT gene, it is possible to detect Mesp1-expressing cells. Mesp1 is a transcriptional factor temporarily expressing in endothelium of the blood vessel in a part of lateral plate mesoderm and heart cells, and is a determinant that is important for the formation of heart. This strain is useful for understanding of the mechanisms underlying cardiovascular development. Homozygous mutant mice are embryonic lethal. Mesp1 遺伝子座 cre recombinase が発現。 <a href='https://brc.riken.jp/mus/pcr01145'>Genotyping protocol -PCR-</a> Mesp1-cre Mesp1-cre Cre/loxP system 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Development, 126, 3437-3447 (1999). 第7番染色体にのっており、tyrosinase gene と近いので、アルビノと交配すると、targeted allele を持つものは、茶か黒になる (ほとんどアグーチ）。交配に供したICRの購入元：クレア（MCH) Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://www.nig.ac.jp/nig/research/organization-top/organization/saga" target="_blank">Saga Lab HP</A> Heterozygote x Wild-type [or Crossing to Jcl:MCH(ICR)]. Homozygous mutant mice are embryonic lethal. Natl. Inst. Genet. 開発年：1998年 開発者：相賀裕美子先生 機関名：国立医薬品食品衛生研究所 C（3〜6か月） 国立遺伝学研究所 TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] C (3-6 months) Developed by Yumiko Sga, National Institute of Health Sciences in 1998. A Cre gene and pgk-neo cassette were ligated in frame at a NcoI site located at the translational initiation site of Mesp1 gene. ICR mixed background. phage P1 Cre recombinase, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Mesp1 genomic DNA 相賀　裕美子 Yumiko SAGA RBRC01145 Developmental Biology Research 開発年：1998年開発者：相賀裕美子先生機関名：国立医薬品食品衛生研究所 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 126, 3437-3447 (1999). true 第7番染色体にのっており、tyrosinase gene と近いので、アルビノと交配すると、targeted allele を持つものは、茶か黒になる (ほとんどアグーチ）。 交配に供したICRの購入元：クレア（MCH) ICR.Cg-Mesp1<tm2(cre)Ysa>/YsaRbrc ICR.Cg-Mesp1<tm2(cre)Ysa>/YsaRbrc <A HREF="https://mus.brc.riken.jp/ja/mouse_of_month/feb_2006_mm" target="_blank">Mouse of the Month Feb 2006</A><br><a href="https://mus.list.brc.riken.jp/ja/wp-content/pdf/01145_GB.pdf">Genetic Background</a>